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BIO 139 - Techniques in Molecular BiologySemester Hours: 4Periodically This course covers routine and advanced techniques in molecular biology. Theory underlying the techniques is covered in lectures. Techniques are learned in the context of interrelated experiments that address a single research problem. Techniques to be covered include DNA and RNA isolations, acrylamide and agarose gel electrophoresis, recombinant DNA techniques, Southern and Northern blot analysis, PCR, protein isolation and characterization by Western blot analysis and DNA and protein database analysis. Students are required to prepare individual or group-based oral presentations using appropriate computer-based technologies. Recommended for students interested in biotechnology or research in genetics and molecular biology. (2 hours lecture, 4 hours laboratory). Prerequisite(s)/Course Notes: Junior class standing and at least one of the following courses: BIO 135 , 137 ; BCHM 162 , 163 .
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