BCHM 173 - Experimental BiochemistrySemester Hours: 3
This course covers routine and advanced techniques in biochemistry. Theory underlying the techniques is covered in lectures. Techniques are learned in the context of interrelated experiments that address a single research problem. Techniques to be covered include homology modeling of proteins, site-directed mutagenesis, bacterial transformation and microbiological techniques, recombinant protein expression and chromatographic purification, polyacrylamide gel electrophoresis, PCR, enzyme characterization by initial rate kinetics, and UV-vis spectrophotometry. Students present research results in written and oral formats (1 hour lecture, 6 hours laboratory).
BCHM 162 ; CHEM 109 . No liberal arts credit. Lab fee additional.
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