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Apr 16, 2024
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BIOL 167 - Techniques in Cell BiologySemester Hours: 4
Once thought to be the purview only of cell and molecular biologists, molecular and cellular methods are now commonly used to solve problems in all sub-disciplines of biology, including conservation, ecology and physiology. Discussion and application of the methods used during the study of membrane trafficking and cell signaling in human cell culture. Topics include CRISPR-Cas9 genome editing, RNA interference, protein structure and function, mechanisms of protein regulation, protein trafficking and expression, and cell signaling. This course provides training in industry-applicable techniques like ELISA (enzyme-linked substrate immunosorbence assay) and confocal immunofluorescence microscopy. Students will design independent experimental projects while
developing scientific writing and presentation skills. Extensive critical reading of primary literature is required. 1 hour lecture, 5 hours lab.
Prerequisite(s)/Course Notes:
BIOL 123 (or BIO 137) or BIOL 122 (or BIO 135); one Laboratory Skills Course (BIOL 126, 127, 128, or 129) or BIO 013, 014 and 015. Fulfills one of the upper-level two lab courses required for a biology major. Lab fee additional.
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