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Nov 27, 2024
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BIO 238 - Modern Laboratory Techniques
Semester Hours: 4
Periodically
Once thought to be the purview only of cell and molecular biologists, molecular and cellular methods are now commonly used to solve problems in all sub-disciplines of biology, including conservation, ecology and physiology. This course provides students the opportunity to learn and practice techniques commonly used in biology, biochemistry, and genetics laboratories while solving specific biological problems. The specific techniques learned may vary depending on the biological problems that are addressed each semester. Polymerase chain reaction (PCR)-related techniques, recombinant DNA methods for prokaryotes and eukaryotes, cell culture, DNA, RNA and protein purification, molecular separation methods including gel electrophoresis, immunoassays, light-level microscopy (phase contrast, fluorescence), protein activity assays, DNA sequencing, nucleic acid and protein hybridizations, and genomic database searches are examples of the techniques that may be learned.
Prerequisite(s)/Course Notes:
An undergraduate course in cell biology or biochemistry is strongly recommended. Students will be expected to work in teams and complete some experimental protocols outside of regularly scheduled class sessions. No credit for BIO 238 if BIO 139 is on undergraduate transcript. (Formerly Animal Cell Culture.)
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